Affordable Access

Access to the full text

Synthetic and biological approaches to map substrate specificities of proteases

Authors
  • Chen, Shiyu1
  • Yim, Joshua J.1
  • Bogyo, Matthew1, 1
  • 1 Stanford University School of Medicine, USA , (United States)
Type
Published Article
Journal
Biological Chemistry
Publisher
Walter de Gruyter GmbH
Publication Date
Oct 22, 2019
Volume
401
Issue
1
Pages
165–182
Identifiers
DOI: 10.1515/hsz-2019-0332
Source
De Gruyter
Keywords
License
Yellow

Abstract

Proteases are regulators of diverse biological pathways including protein catabolism, antigen processing and inflammation, as well as various disease conditions, such as malignant metastasis, viral infection and parasite invasion. The identification of substrates of a given protease is essential to understand its function and this information can also aid in the design of specific inhibitors and active site probes. However, the diversity of putative protein and peptide substrates makes connecting a protease to its downstream substrates technically difficult and time-consuming. To address this challenge in protease research, a range of methods have been developed to identify natural protein substrates as well as map the overall substrate specificity patterns of proteases. In this review, we highlight recent examples of both synthetic and biological methods that are being used to define the substrate specificity of protease so that new protease-specific tools and therapeutic agents can be developed.

Report this publication

Statistics

Seen <100 times