Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells.

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Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells.

Authors
  • S Detke
  • J L Stein
  • G S Stein
Source
PMC
License
Green

Abstract

Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.

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