Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells.

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Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells.

Publication Date
May 01, 1978
Source
PMC
License
Unknown

Abstract

Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.

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