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Synthesis of biotin labelled cap analogue--incorporable into mRNA transcripts and promoting cap-dependent translation.

Authors
  • Jemielity, Jacek
  • Lukaszewicz, Maciej
  • Kowalska, Joanna
  • Czarnecki, Jakub
  • Zuberek, Joanna
  • Darzynkiewicz, Edward
Type
Published Article
Journal
Organic & Biomolecular Chemistry
Publisher
The Royal Society of Chemistry
Publication Date
Nov 21, 2012
Volume
10
Issue
43
Pages
8570–8574
Identifiers
DOI: 10.1039/c2ob26060c
PMID: 22832840
Source
Medline
License
Unknown

Abstract

Analogues of the eukaryotic messenger RNA 5' end (m(7)G cap) are useful tools for studying mRNA fate and serve as reagents for in vitro preparation of 5' capped mRNAs. We designed a biotin-labeled dinucleotide cap analogue that can be incorporated into transcripts to produce 5'-capped and biotinylated mRNAs which retain their biological functionality and may be employed for biotin-(strept)avidin technologies.

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