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Synchrotron X-ray-induced photoreduction of ferric myoglobin nitrite crystals gives the ferrous derivative with retention of the O-bonded nitrite ligand.

Authors
  • Yi, Jun
  • Orville, Allen M
  • Skinner, John M
  • Skinner, Michael J
  • Richter-Addo, George B
Type
Published Article
Journal
Biochemistry
Publisher
American Chemical Society
Publication Date
Jul 27, 2010
Volume
49
Issue
29
Pages
5969–5971
Identifiers
DOI: 10.1021/bi100801g
PMID: 20568729
Source
Medline
License
Unknown

Abstract

Exposure of a single crystal of the nitrite adduct of ferric myoglobin (Mb) at 100 K to high-intensity synchrotron X-ray radiation resulted in changes in the UV-vis spectrum that can be attributed to reduction of the ferric compound to the ferrous derivative. We employed correlated single-crystal spectroscopy with crystallography to further characterize this photoproduct. The 1.55 A resolution crystal structure of the photoproduct reveals retention of the O-binding mode for binding of nitrite to the iron center. The data are consistent with cryogenic generation and trapping, at 100 K, of a ferrous d(6) Mb(II)(ONO)* complex by photoreduction of the ferric precursor crystals using high-intensity X-ray radiation.

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