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Symmetry breaking in the female germline cyst.

Authors
  • Nashchekin, Dmitry
  • Busby, L
  • Jakobs, Maximilian
  • Squires, I
  • St Johnston, Daniel
Publication Date
Nov 12, 2021
Source
Apollo - University of Cambridge Repository
Keywords
Language
English
License
Green
External links

Abstract

In mammals and flies, only one cell in a multicellular female germline cyst becomes an oocyte, but how symmetry is broken to select the oocyte is unknown. Here, we show that the microtubule (MT) minus end-stabilizing protein Patronin/CAMSAP marks the future Drosophila oocyte and is required for oocyte specification. The spectraplakin Shot recruits Patronin to the fusome, a branched structure extending into all cyst cells. Patronin stabilizes more MTs in the cell with the most fusome material. Our data suggest that this weak asymmetry is amplified by Dynein-dependent transport of Patronin-stabilized MTs. This forms a polarized MT network, along which Dynein transports oocyte determinants into the presumptive oocyte. Thus, Patronin amplifies a weak fusome anisotropy to break symmetry and select one cell to become the oocyte.

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