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SVIP is a molecular determinant of lysosomal dynamic stability, neurodegeneration and lifespan.

Authors
  • Johnson, Alyssa E
  • Orr, Brian O
  • Fetter, Richard D
  • Moughamian, Armen J
  • Primeaux, Logan A
  • Geier, Ethan G
  • Yokoyama, Jennifer S
  • Miller, Bruce L
  • Davis, Graeme W
Publication Date
Jan 21, 2021
Source
eScholarship - University of California
Keywords
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Unknown
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Abstract

Missense mutations in Valosin-Containing Protein (VCP) are linked to diverse degenerative diseases including IBMPFD, amyotrophic lateral sclerosis (ALS), muscular dystrophy and Parkinson's disease. Here, we characterize a VCP-binding co-factor (SVIP) that specifically recruits VCP to lysosomes. SVIP is essential for lysosomal dynamic stability and autophagosomal-lysosomal fusion. SVIP mutations cause muscle wasting and neuromuscular degeneration while muscle-specific SVIP over-expression increases lysosomal abundance and is sufficient to extend lifespan in a context, stress-dependent manner. We also establish multiple links between SVIP and VCP-dependent disease in our Drosophila model system. A biochemical screen identifies a disease-causing VCP mutation that prevents SVIP binding. Conversely, over-expression of an SVIP mutation that prevents VCP binding is deleterious. Finally, we identify a human SVIP mutation and confirm the pathogenicity of this mutation in our Drosophila model. We propose a model for VCP disease based on the differential, co-factor-dependent recruitment of VCP to intracellular organelles.

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