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Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica.

Authors
  • Yu, Aiqun1
  • Zhao, Yu1
  • Li, Jian1
  • Li, Shenglong1
  • Pang, Yaru1
  • Zhao, Yakun1
  • Zhang, Cuiying1
  • Xiao, Dongguang1
  • 1 State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China. , (China)
Type
Published Article
Journal
MicrobiologyOpen
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jul 01, 2020
Volume
9
Issue
7
Identifiers
DOI: 10.1002/mbo3.1051
PMID: 32342649
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Fatty acid ethyl esters (FAEEs) are fatty acid-derived molecules and serve as an important form of biodiesel. The oleaginous yeast Yarrowia lipolytica is considered an ideal host platform for the production of fatty acid-derived products due to its excellent lipid accumulation capacity. In this proof-of-principle study, several metabolic engineering strategies were applied for the overproduction of FAEE biodiesel in Y. lipolytica. Here, chromosome-based co-overexpression of two heterologous genes, namely, PDC1 (encoding pyruvate decarboxylase) and ADH1 (encoding alcohol dehydrogenase) from Saccharomyces cerevisiae, and the endogenous GAPDH (encoding glyceraldehyde-3-phosphate dehydrogenase) gene of Y. lipolytica resulted in successful biosynthesis of ethanol at 70.8 mg/L in Y. lipolytica. The engineered Y. lipolytica strain expressing the ethanol synthetic pathway together with a heterologous wax ester synthase (MhWS) exhibited the highest FAEE titer of 360.8 mg/L, which is 3.8-fold higher than that of the control strain when 2% exogenous ethanol was added to the culture medium of Y. lipolytica. Furthermore, a synthetic microbial consortium comprising an engineered Y. lipolytica strain that heterologously expressed MhWS and a S. cerevisiae strain that could provide ethanol as a substrate for the production of the final product in the final engineered Y. lipolytica strain was created in this study. Finally, this synthetic consortium produced FAEE biodiesel at a titer of 4.8 mg/L under the optimum coculture conditions. © 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

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