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A sustainable amperometric biosensor for the analysis of ascorbic, benzoic, gallic and kojic acids through catechol detection. Innovation and signal processing.

  • Casanova, Ana1
  • Cuartero, María2
  • Alacid, Yolanda1
  • Almagro, Carmen M1
  • García-Cánovas, Francisco3
  • García, María S1
  • Ortuño, Joaquín A1
  • 1 Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Murcia E-30100, Spain. [email protected] , (Spain)
  • 2 Department of Chemistry, School of Engineering Science in Chemistry, Biotechnology and Healthcare, KTH Royal Institute of Technology, 10044, Stockholm, Sweden. [email protected] , (Sweden)
  • 3 Department of Biochemistry and Molecular Biology-A, Faculty of Biology, University of Murcia, Murcia E-30100, Spain. , (Spain)
Published Article
The Analyst
The Royal Society of Chemistry
Publication Date
Apr 02, 2020
DOI: 10.1039/c9an02523e
PMID: 32239042


In this work, we present a new catechol amperometric biosensor fabricated on the basis of naturally available enzymes in common mushrooms. The biosensor response mechanism comprises the reduction of the quinone exclusively produced in the oxidation of the catechol present in the sample, which is catalyzed by tyrosinase enzyme. The new catechol biosensor has demonstrated excellent analytical performance at increasing catechol concentrations in the sample solution, which includes superior reproducibility for several electrodes and long-term stability. On top of that, the biosensing element used in the fabrication is a sustainable material, of low-cost and presents an excellent lifetime of years. Whether the catechol biosensor is operating in the presence of a compound influencing the reactions underlying the amperometric response (such as ascorbic, benzoic, gallic and kojic acids), this serves as an analytical platform to detect these compounds in real samples. Particularly, we introduce herein for the first time different treatments to process the current signal of the biosensor pursuing the linearity needed for the analytical application in real samples. In this sense, the catechol biosensor has been successfully applied to the detection of benzoic, gallic and kojic acids in juices, teas and cosmetic products, respectively.

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