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Sustainable Ethanol Production From Sugarcane Molasses by Saccharomyces cerevisiae Immobilized on Chitosan-Coated Manganese Ferrite

Authors
  • Núñez Caraballo, Arianna1
  • Iliná, Anna1
  • Ramos González, Rodolfo1
  • Aguilar, Cristóbal N.2
  • Michelena Álvarez, Georgina3
  • Flores Gallegos, Adriana Carolina2
  • Sandoval-Cortés, José2
  • Aguilar-Gonzalez, Miguel A.4
  • Soto-Cruz, Nicolas Oscar5
  • García García, José Daniel1
  • Martínez-Hernández, José L.1
  • 1 Nanobioscience Group, Autonomous University of Coahuila, Saltillo , (Mexico)
  • 2 Group of Bioprocesses and Bioproducts, Food Research Department, Autonomous University of Coahuila, Saltillo , (Mexico)
  • 3 Instituto Cubano de Investigaciones de Los Derivados de la Caña de Azúcar (ICIDCA), Ciudad de la Habana , (Cuba)
  • 4 Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Unidad Saltillo, Ramos Arizpe , (Mexico)
  • 5 Chemistry and Biochemistry, National Technology of Mexico/Instituto Tecnológico de Durango, Durango , (Mexico)
Type
Published Article
Journal
Frontiers in Sustainable Food Systems
Publisher
Frontiers Media S.A.
Publication Date
Jun 07, 2021
Volume
5
Identifiers
DOI: 10.3389/fsufs.2021.683170
Source
Frontiers
Keywords
Disciplines
  • Sustainable Food Systems
  • Original Research
License
Green

Abstract

The interaction between nanostructures and yeast cells, as well as the description of the effect of nanoparticles in ethanol production are open questions in the development of this nanobiotechnological process. The objective of the present study was to evaluate the ethanol production by Saccharomyces cerevisiae in the free and immobilized state on chitosan-coated manganese ferrite, using cane molasses as a carbon source. To obtain the chitosan-coated manganese ferrite, the one-step coprecipitation method was used. The nanoparticles were characterized by X-ray diffraction obtaining the typical diffraction pattern. The crystal size was calculated by the Scherrer equation as 15.2 nm. The kinetics of sugar consumption and ethanol production were evaluated by HPLC. With the immobilized system, it was possible to obtain an ethanol concentration of 56.15 g/L, as well as the total sugar consumption at 24 h of fermentation. Productivity and yield in this case were 2.3 ± 0.2 g/(L * h) and 0.28 ± 0.03, respectively. However, at the same time in the fermentation with free yeast, 39.1 g/L were obtained. The total consumption of fermentable sugar was observed only after 42 h, reaching an ethanol titer of 50.7 ± 3.1, productivity and yield of 1.4 ± 0.3 g/(L * h) and 0.25 ± 0.4, respectively. Therefore, a reduction in fermentation time, higher ethanol titer and productivity were demonstrated in the presence of nanoparticles. The application of manganese ferrite nanoparticles shows a beneficial effect on ethanol production. Research focused on the task of defining the mechanism of their action and evaluation of the reuse of biomass immobilized on manganese ferrite in the ethanol production process should be carried out in the future.

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