Pseudomonas aeruginosa is a germ of hospitalism responsible for nosocomial infections; it is naturally resistant to many antibiotics and has a high susceptibility to the acquisition of acquiring new resistance. The observation of strains highly resistant to antibiotics, has led us to look for possible alternative therapeutics. This study was a descriptive and cross-sectional one, conducted from October 2010 to March 2011. All patients hospitalized for at least 48 hours and showing sign of infection were included after obtaining their consent. Forty nine of 150 samples were positive to the cultivation of Pseudomonas aerug-inosa showing a prevalence of 32.66%. For the antibiotic susceptibility, we obtain amikacin 57.14%, netilmicin 59.20%, ceftazidime 52.60%, imipenem 33%, colistin 97.95%, and ciprofloxacin 51%. Seven strains were resistant to all antibiotics tested other than colistin. One strain was resistant to colistin. Colistin retains high sensitivity to Pseudomonas aeruginosa. However, there are some strains multiresistant to antibiotics .