This retrospective study analyzed the survivorship of DKS instrumentation and the clinical outcomes in 185 patients with spondylolisthesis. These patients were treated with Zielke DKS instrumentation for a mean followup period of 3.5 years. Eight (4.3%) patients had late removal of implants, 25 (14%) had rod breakage, three (1.7%) had screw breakage, and 16 (8.7%) had nut loosening. The survivor rate of DKS instrumentation was 96% within 3 months after operation, 80% at 2 years, and 61% at 5 years after surgery. One hundred sixty-three (88%) patients had solid posterolateral fusion, and 167 (90%) patients had good to excellent results. Adjacent instability developed in 18 (9.7%) patients. Although Zielke DKS instrumentation has a smaller rod and relatively insecure locking system between the rod and screw, it is an effective implant for the treatment of spondylolisthesis.