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Survival and differentiation of neuroepithelial stem cells on chitosan bicomponent fibers.

Authors
  • Fang, Peng
  • Gao, Qing
  • Liu, Wen-Jing
  • Qi, Xiao-Xia
  • Li, Gui-Bao
  • Zhang, Jing
  • Li, Zhen-Hua
  • Sun, Jin-Long
  • Sun, Jin-Hao
  • Gao, Ying-Mao
Type
Published Article
Journal
The Chinese journal of physiology
Publication Date
Aug 31, 2010
Volume
53
Issue
4
Pages
208–214
Identifiers
PMID: 21793330
Source
Medline
License
Unknown

Abstract

Chitosan is a popular biomaterial used in tissue engineering. Fibers of chitosan could provide a favorable anatomical substrate for cell growth which provides a promising application for axonal regeneration during peripheral injury. Neuroepithelial stem cells (NEPs) are the most primitive neural stem cells with multipotential for neuronal and glia differentiation. To assess the biocompatibility between NEPs and chitosan fibers, and to explore whether the NEPs have the ability to differentiation on chitosan fibers, NEPs were harvested from the neural tube and seeded on chitosan fibers in in vitro culture. The biocompatibility of chitosan fibers was tested by MTT assays. The growth and survival were observed by light and scanning electronic microscope at different times in culture. And, the differentiation of NEPs was examined by immunocytochemical staining. The results indicated that NEPs could grow on the chitosan fibers and attach firmly to the surface of fibers. On chitosan fibers, NEPs could differentiate into neurons and glia. Our study demonstrated that chitasan fibers had a good biocompatibility with NEPs which affords a potential alternative for the repair of peripheral nerve injury.

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