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Surface-enhanced Raman scattering (SERS)–based immunosystem for ultrasensitive detection of the 90K biomarker

Authors
  • Gallo, Valentina1
  • Lai, Antonia2
  • Pasquo, Alessandra2
  • Almaviva, Salvatore2
  • Iacobelli, Stefano1, 3
  • Persichetti, Luca4
  • Capellini, Giovanni4
  • Antonini, Giovanni1, 4
  • 1 Interuniversity Consortium INBB - Biostructures and Biosystems National Institute,
  • 2 ENEA,
  • 3 Mediapharma srl, 66100 Chieti, Italy
  • 4 University Roma Tre,
Type
Published Article
Journal
Analytical and Bioanalytical Chemistry
Publisher
Springer-Verlag
Publication Date
Sep 02, 2020
Volume
412
Issue
27
Pages
7659–7667
Identifiers
DOI: 10.1007/s00216-020-02903-2
PMID: 32875368
PMCID: PMC7533257
Source
PubMed Central
Keywords
License
Unknown

Abstract

The research and the individuation of tumour markers in biological fluids are currently one of the main tools to support diagnosis, prognosis, and monitoring of the therapeutic response in oncology. Although the identification of tumour markers in asymptomatic patients is crucial for early diagnosis, its application is still limited by the relatively low sensitivity and the complexity of existing methods (i.e. ELISA, mass spectrometry). We developed an easy, fast, and ultrasensitive surface-enhanced Raman scattering (SERS)–based system, for the detection and quantitation of the LGALS3BP (90K) biomarker that was used as a model, based on the development of antibody-functionalized nanostructured gold surfaces. The detection system was effective for the ultrasensitive detection and characterization of samples of different biochemical compositions. In conclusion, this work could provide the foundation for the development of a medical diagnostic device with the highest predictive power when compared with the methods currently used in cancer diagnostics. Electronic supplementary material The online version of this article (10.1007/s00216-020-02903-2) contains supplementary material, which is available to authorized users.

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