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Surface supported bilayer platform for studies of lateral association of proteins in membranes (Mini Review).

Authors
  • Merzlyakov, Mikhail
  • Li, Edwin
  • Hristova, Kalina
Type
Published Article
Journal
Biointerphases
Publisher
American Institute of Physics
Publication Date
Jun 01, 2008
Volume
3
Issue
2
Identifiers
DOI: 10.1116/1.2912096
PMID: 20408673
Source
Medline
License
Unknown

Abstract

Here, the authors review how surface supported bilayers can be engineered and how Forster resonance energy transfer (FRET) can be used to quantify interactions between transmembrane peptides in these bilayers. The requirements for the surface supported platform are (1) lateral mobility of the peptides, (2) transmembrane orientation of the peptides, and (3) capabilities for FRET measurements. To satisfy these requirements, a new assembly method, termed "directed assembly" was developed. This assembly method could have broad utility in basic studies of proteins in membranes and in biotechnological applications.

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