Affordable Access

Surface plasmon resonance analysis for the screening of anti-prion compounds.

Authors
  • Kawatake, Satoshi
  • Nishimura, Yuki
  • Sakaguchi, Suehiro
  • Iwaki, Toru
  • Doh-ura, Katsumi
Type
Published Article
Journal
Biological & pharmaceutical bulletin
Publication Date
May 01, 2006
Volume
29
Issue
5
Pages
927–932
Identifiers
PMID: 16651721
Source
Medline
License
Unknown

Abstract

The interaction of anti-prion compounds and amyloid binding dyes with a carboxy-terminal domain of prion protein (PrP121-231) was examined using surface plasmon resonance (SPR) and compared with inhibition activities of abnormal PrP formation in scrapie-infected cells. Most examined compounds had affinities for PrP121-231: antimalarials had low affinities, whereas Congo red, phthalocyanine and thioflavin S had high affinities. The SPR binding response correlated with the inhibition activity of abnormal PrP formation. Several drugs were screened using SPR to verify the findings: propranolol was identified as a new anti-prion compound. This fact indicates that drug screenings by this assay are useful.

Report this publication

Statistics

Seen <100 times