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Suppression of epithelial folding at actomyosin-enriched compartment boundaries downstream of Wingless signalling in Drosophila.

Authors
  • Urbano, Jose M1
  • Naylor, Huw W1
  • Scarpa, Elena1
  • Muresan, Leila1, 2
  • Sanson, Bénédicte3
  • 1 Department of Physiology, Development and Neuroscience, University of Cambridge, Anatomy Building, Downing Street, Cambridge, CB2 3DY, UK.
  • 2 Cambridge Advanced Imaging Centre, University of Cambridge, Anatomy Building, Downing Street, Cambridge, CB2 3DY, UK.
  • 3 Department of Physiology, Development and Neuroscience, University of Cambridge, Anatomy Building, Downing Street, Cambridge, CB2 3DY, UK [email protected]
Type
Published Article
Journal
Development
Publisher
The Company of Biologists
Publication Date
Apr 24, 2018
Volume
145
Issue
8
Identifiers
DOI: 10.1242/dev.155325
PMID: 29691225
Source
Medline
Keywords
License
Unknown

Abstract

Epithelial folding shapes embryos and tissues during development. Here, we investigate the coupling between epithelial folding and actomyosin-enriched compartmental boundaries. The mechanistic relationship between the two is unclear, because actomyosin-enriched boundaries are not necessarily associated with folds. Also, some cases of epithelial folding occur independently of actomyosin contractility. We investigated the shallow folds called parasegment grooves that form at boundaries between anterior and posterior compartments in the early Drosophila embryo. We demonstrate that formation of these folds requires the presence of an actomyosin enrichment along the boundary cell-cell contacts. These enrichments, which require Wingless signalling, increase interfacial tension not only at the level of the adherens junctions but also along the lateral surfaces. We find that epithelial folding is normally under inhibitory control because different genetic manipulations, including depletion of the Myosin II phosphatase Flapwing, increase the depth of folds at boundaries. Fold depth correlates with the levels of Bazooka (Baz), the Par-3 homologue, along the boundary cell-cell contacts. Moreover, Wingless and Hedgehog signalling have opposite effects on fold depth at the boundary that correlate with changes in Baz planar polarity.

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