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Supplementation of the cultivation media with B-group vitamins enhances lovastatin biosynthesis by Aspergillus terreus.

Authors
Type
Published Article
Journal
Journal of Biotechnology
0168-1656
Publisher
Elsevier
Publication Date
Volume
127
Issue
2
Pages
258–268
Identifiers
PMID: 16887228
Source
Medline
License
Unknown

Abstract

The impact of the supplementation of cultivation media with B-group vitamins on the biosynthesis of lovastatin (mevinolinic acid) by Aspergillus terreus ATCC20542 was investigated. A hypothesis was formulated that as the biosynthesis of lovastatin requires a high throughput of coenzymes in the cells, the application of its precursors in the form of B-group vitamins might positively influence the process. In a nitrogen-deficient medium the B-group vitamins, both single, especially nicotinamide, pyridoxine and calcium D-pantothenate, and a mixture of thiamine, riboflavin, pyridoxine, calcium d-pantothenate and nicotinamide increased the efficiency of lovastatin biosynthesis. The vitamin supplementation also increased both volumetric and specific production rates of mevinolinic acid, especially before 80 h of the process, when no lactose limitation had been observed yet.

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