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Super-resolution microscopy reveals LINC complex recruitment at nuclear indentation sites.

Authors
  • Versaevel, Marie
  • Braquenier, Jean-Baptiste
  • Riaz, Maryam
  • Grevesse, Thomas
  • Lantoine, Joséphine
  • Gabriele, Sylvain
Type
Published Article
Journal
Scientific Reports
Publisher
Springer Nature
Publication Date
Jan 01, 2014
Volume
4
Pages
7362–7362
Identifiers
DOI: 10.1038/srep07362
PMID: 25482017
Source
Medline
License
Unknown

Abstract

Increasing evidences show that the actin cytoskeleton is a key parameter of the nuclear remodeling process in response to the modifications of cellular morphology. However, detailed information on the interaction between the actin cytoskeleton and the nuclear lamina was still lacking. We addressed this question by constraining endothelial cells on rectangular fibronectin-coated micropatterns and then using Structured Illumination Microscopy (SIM) to observe the interactions between actin stress fibers, nuclear lamina and LINC complexes at a super-resolution scale. Our results show that tension in apical actin stress fibers leads to deep nuclear indentations that significantly deform the nuclear lamina. Interestingly, indented nuclear zones are characterized by a local enrichment of LINC complexes, which anchor apical actin fibers to the nuclear lamina. Moreover, our findings indicate that nuclear indentations induce the formation of segregated domains of condensed chromatin. However, nuclear indentations and condensed chromatin domains are not irreversible processes and both can relax in absence of tension in apical actin stress fibers.

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