Affordable Access

deepdyve-link
Publisher Website

Sulfonyl fluoride analogues as activity-based probes for serine proteases.

Authors
  • Shannon, D Alexander
  • Gu, Christian
  • McLaughlin, Christopher J
  • Kaiser, Markus
  • van der Hoorn, Renier A L
  • Weerapana, Eranthie
Type
Published Article
Journal
ChemBioChem
Publisher
Wiley (John Wiley & Sons)
Publication Date
Nov 05, 2012
Volume
13
Issue
16
Pages
2327–2330
Identifiers
DOI: 10.1002/cbic.201200531
PMID: 23008217
Source
Medline
License
Unknown

Abstract

Enriched with fluoride: To expand on the available tools to interrogate proteases, we explored sulfonyl fluorides as activity-based probes. An alkyne-tagged sulfonyl fluoride covalently modifies members of the S1 family of serine proteases. By applying click chemistry, avidin enrichment and mass spectrometry, we can enrich and identify active endogenous serine proteases from a complex proteome.

Report this publication

Statistics

Seen <100 times