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Sugar Uptake in Lily Pollen : A PROTON SYMPORT.

Authors
  • 1
  • 1 Department of Biochemistry, University of Geneva, Geneva, Switzerland. , (Switzerland)
Type
Published Article
Journal
PLANT PHYSIOLOGY
0032-0889
Publisher
American Society of Plant Biologists
Publication Date
Volume
67
Issue
4
Pages
793–796
Identifiers
PMID: 16661756
Source
Medline
License
Unknown

Abstract

The data presented here are consistent with a proton-sugar co-transport in germinated pollen of Lilium longiflorum Thunb. Optimal uptake occurs at pH 5.0. A K(m) of 1.7 to 1.8 millimolar is obtained from the initial rate of pH change induced by sucrose uptake as well as from uptake of [U-(14)C]-sucrose. The energy of activation is - 11 kilocalories mole(-1). The effect of several inhibitors and sugar competitors on [U-(14)C]sucrose and d-[U-(14)C] glucose uptake is given. The possibility of hydrolysis of sucrose prior to its transport into the pollen tube has been considered and reasons for choosing a sucrose-type uptake are presented. The possible in vivo significance of this co-transport process during pollen germination is discussed. Germinated pollen has features to recommend it as an experimental system of choice for studies of sugar uptake.

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