Lateral ankle stability and how it changes in different footwear has been investigated for years. Research, however, has shown a lack of reliability or sensitivity of available methodologies. This study aimed to evaluate the test-retest reliability and sensitivity of a novel lateral stability protocol, the Submaximal Lateral Shuffle Test (SLST). We recruited 11 and 40 participants to assess reliability and sensitivity of the SLST, respectively. Participants performed the SLST in footwear that differed in collar height and upper stiffness. ICC values showed good to excellent reliability in peak ankle angles and moments, ground reaction forces, impulses, stance time, and performance time. Significantly lower peak inversion and adduction angles and lower medio-lateral push off peak forces were found in the high cut shoes compared to the low cut shoes. The medio-lateral landing peak force showed lower forces in the high cut shoes. The smallest worthwhile change indicated meaningful differences in 70.0-82.5% of participants for inversion, adduction, medio-lateral landing peak, and push off peak forces. These results, however, were not systematic such that there was not a consistent direction of the difference for all participants. In conclusion, the SLST is a promising protocol to further investigate lateral stability in footwear.