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Sublethal effects of carbendazim in Jenynsia multidentata detected by a battery of molecular, biochemical and genetic biomarkers.

Authors
  • Götte, Jesica Y1
  • Carrizo, Juan Cruz2
  • Panzeri, Ana M3
  • Amé, M Valeria2
  • Menone, Mirta L4
  • 1 Facultad de Ciencias Exactas y Naturales, Departamento de Ciencias Marinas, Mar Del Plata, Argentina. , (Argentina)
  • 2 Universidad Nacional de Córdoba - CONICET, Facultad de Ciencias Químicas, Dto. Bioquímica Clínica-CIBICI, Córdoba, Argentina. , (Argentina)
  • 3 Laboratorio de Ecotoxicología, Instituto de Investigaciones Marinas y Costeras (IIMYC), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Mar Del Plata (CONICET- UNMdP), Dean Funes 3350, 7600, Mar Del Plata, Buenos Aires, Argentina. , (Argentina)
  • 4 Laboratorio de Ecotoxicología, Instituto de Investigaciones Marinas y Costeras (IIMYC), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Mar Del Plata (CONICET- UNMdP), Dean Funes 3350, 7600, Mar Del Plata, Buenos Aires, Argentina. Electronic address: [email protected] , (Argentina)
Type
Published Article
Journal
Ecotoxicology and Environmental Safety
Publisher
Elsevier
Publication Date
Aug 20, 2020
Volume
205
Pages
111157–111157
Identifiers
DOI: 10.1016/j.ecoenv.2020.111157
PMID: 32829211
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The fungicide carbendazim (CBM) has been applied all around the world but its potential adverse effects other than its recognized activity as endocrine disruptor in non target organisms have been scarcely studied. The aims of this work were (1) to use a battery of biomarkers that can reflect potential negative effects such as oxidative stress, genotoxicity, neurotoxicity or altered immune response; and (2) to examine biomarkers of detoxification by analyzing the gene expression of cytochrome P4501A1 (CYP1A1) and the multi-xenobiotic resistance protein P-glycoprotein (P-gp) in the freshwater fish Jenynsia multidentata exposed to environmentally relevant concentrations of CBM during 24 h. Fish exposed to 5 μg/L showed inhibition of GST activity and an increase of TBARs contents in gills, the organ of direct contact with waterborne contaminants. Genotoxicity - measured in peripheral blood-was evidenced by the increases of micronuclei frequency when fish were exposed to 5, 10 and 100 μg/L CBM and of nuclear abnormalities (NA) frequency at 0.05, 0.5, 5, 10 and 100 μg/L CBM. The expression inhibition of interleukin (IL-1β) and tumor necrosis factor a (TNF-α) at 10, and 5 and 10 μg/L CBM, respectively, indicated an altered immune response. The expression of CYP1A1 was down regulated in liver at 10 μg/L and of P-gp at 5 μg/L CBM, indicating a possible slow on CBM metabolization. On the other hand, in gills CYP1A1 decreased at 5 and 10 μg/L while P-gp was induced at 5 and 100 μg/L CBM. Overall, most of these significant effects were detected below 10 μg/L CBM, in a range of realistic concentrations in aquatic ecosystems worldwide. Copyright © 2020. Published by Elsevier Inc.

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