In order to relate EMG signs of localized muscle fatigue to subjectively perceived muscle fatigue, a study was undertaken on 20 healthy volunteers exposing their right trapezius muscle by raising the arm to 90 degrees of abduction. Every person performed two contractions: 0 kg hand load during 5 min and 2 kg hand load during 2.5 min. Surface EMG was recorded and analysed with respect to RMS amplitude and mean power frequency (MPF). Subjective muscle fatigue was estimated with a psychophysical rating scale (Borg's CR-10 Scale). At high load level we found a significant correlation between MPF and the CR-scores (r = -0.46), but at low load level there was no correlation. At high load level there was a linear decrease of MPF with increasing load dose, but at low load level the MPF did not change, despite significant subjective fatigue. There was a linear rise of the CR-score with increasing load dose, more pronounced at high load level. It was concluded, that at low load levels common in working life, MPF and subjective scores seem to provide different fatigue information. Moreover, the MPF did not seem to work as a valid estimator of muscle fatigue at this low load level. Caution is recommended if it is to be used in static low-load situations.