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A study of process interactions between cell disruption and debris clarification stages in the recovery of yeast intracellular products.

Authors
  • Clarkson, A I
  • Lefevre, P
  • Titchener-Hooker, N J
Type
Published Article
Journal
Biotechnology Progress
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jan 01, 1993
Volume
9
Issue
5
Pages
462–467
Identifiers
PMID: 7764162
Source
Medline
License
Unknown

Abstract

The effects of varying the levels of cell disruption achieved by high-pressure homogenization upon subsequent centrifugal separation of cell debris have been defined by measuring sedimentation velocity distributions of the cell debris. Using this data together with particle size distributions, a computer-based model of a disk stack centrifuge has been used to predict the efficiency of centrifugal separation. These predictions have been compared with both laboratory scale and pilot plant scale experimental results. A similar sedimentation velocity technique has been used to characterize the properties of the flocs formed by addition of poly(ethylene imine) to a borax-clarified homogenate supernatant. The variations of floc settling properties with respect to polymer concentration are defined.

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