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[Study on the effect of monoclonal antibodies on Giardia lamblia in vitro].

Authors
  • Jia, K D
  • Zhu, Y G
  • Lu, S Q
  • Wang, F R
  • Li, G P
  • Wen, P E
Type
Published Article
Journal
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Publication Date
Jan 01, 1994
Volume
12
Issue
1
Pages
56–58
Identifiers
PMID: 8044909
Source
Medline
License
Unknown

Abstract

Monoclonal antibodies (McAbs) were generated by fusion of SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with Giardia lamblia trophozoites. To define the effect of the McAb on Giardia lamblia, trophozoites were subcultured axenically after contact with McAb for 1 h at 37 degrees C. The McAbs 1G5 and 1E2 (both IgG1) were able to inhibit the growth of trophozoite at the rate of 63.4% and 35%, respectively. The inhibitory effect was decreased along with the decreasing quantity of McAb (from dilution 1:45 to 1:225) added to the TYI-S-33 medium. The actions of McAbs could be eliminated by absorption with trophozoites and not be influenced by complement in fresh guinea-pig serum, thus the actions of McAbs appeared to be specific and complement independent.

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