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Studies on the transmembrane migration of fluoride and its effects on proliferation of L-929 fibroblasts (L cells) in vitro.

Authors
  • Kawase, T
  • Suzuki, A
Type
Published Article
Journal
Archives of Oral Biology
Publisher
Elsevier
Publication Date
Jan 01, 1989
Volume
34
Issue
2
Pages
103–107
Identifiers
PMID: 2783043
Source
Medline
License
Unknown

Abstract

A decrease in the pH of the medium facilitated fluoride (F-) influx but depressed its efflux, which is consistent with the hypothesis that simple diffusion of hydrogen fluoride (HF) contributes to F- migration across the cell membrane. Long-term exposure to F- (greater than 1 mM) induced F- accumulation and, as a result, inhibited cell proliferation. In contrast, short-term exposure to F- (greater than 1 mM), followed by careful washing, did not inhibit cell proliferation but rather stimulated it. Moreover, this stimulatory effect was enhanced by 1 microM Al3+ and was inhibited by 1 microM H-7, a specific inhibitor of protein kinase C. Thus F- may stimulate cell proliferation by activating protein kinase C through GTP-binding protein. The stimulatory effect of F- on cell proliferation, which is usually hidden by its inhibitory effect, can be observed by preventing the accumulation of F- in the cytoplasm.

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