Affordable Access

Studies on the synthesis of polynucleotides--synthesis of a dodecaribonucleoside undecaphosphate and a hexadecaribonucleoside pentadecaphosphate.

Type
Published Article
Journal
Scientia Sinica
Publication Date
Jan 01, 1978
Volume
21
Issue
5
Pages
687–697
Identifiers
PMID: 734440
Source
Medline
License
Unknown

Abstract

This paper describes the synthesis of oligoribonucleotides by T4 RNA ligase and their identification. The products are a dodecaribonucleotide CpGpGpA32pCpUpCpGpUpCpCpA and a hexadecaribonucleotide ApUpUpC32pCpUpCpGpUpCpCpA, representing the 3'-end nucleotide sequences of the yeast alanine transfer RNA. Particular emphasis was placed on the conditions for the joining reaction, using oligoribonucleotides of defined sequence as the substrates. CpGpGpA32pCpUpCpGpUpCpCpA was synthesized with 32pCpUpCpGpUpCpCpA as the donor, and CpGpGpA as the acceptor in rather low ratio of acceptor to donor. Both the yield and purity of the joining product were above 90%. 32pCpGpGpA32pCpUpCpGpUpCpCpA was used in turn as the donor, and ApUpUpC as the acceptor for further synthesis of the hexadecaribonucleotide ApUpUpC32pCpGpGpA32pCpUpCpGpUpCpCpA with a yield of 60%.

Report this publication

Statistics

Seen <100 times