Affordable Access

deepdyve-link
Publisher Website

Studies on the singlet oxygen scavenging mechanism of human macular pigment

Authors
  • Li, Binxing
  • Ahmed, Faisal
  • Bernstein, Paul S.
Type
Published Article
Journal
Archives of Biochemistry and Biophysics
Publisher
Elsevier BV
Publication Date
Jan 01, 2010
Volume
504
Issue
1
Pages
56–60
Identifiers
DOI: 10.1016/j.abb.2010.07.024
Source
Elsevier
Keywords
License
Unknown

Abstract

It is thought that direct quenching of singlet oxygen and scavenging free radicals by macular pigment carotenoids is a major mechanism for their beneficial effects against light-induced oxidative stress. Corresponding data from human tissue remains unavailable, however. In the studies reported here, electron paramagnetic resonance (EPR) spectroscopy was used to measure light-induced singlet oxygen generation in post-mortem human macula and retinal pigment epithelium/choroid (RPE/choroid). Under white-light illumination, production of singlet oxygen was detected in RPE/choroid but not in macular tissue, and we show that exogenously added macular carotenoids can quench RPE/choroid singlet oxygen. When the singlet oxygen quenching ability of the macular carotenoids was investigated in solution, it was shown that a mixture of meso-zeaxanthin, zeaxanthin, and lutein in a ratio of 1:1:1 can quench more singlet oxygen than the individual carotenoids at the same total concentration.

Report this publication

Statistics

Seen <100 times