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Studies on satellite nucleoli in human normal and leukemic lymphocytes.

Authors
  • Smetana, K
  • Likovský, Z
  • Ochs, R
  • Novák, J
  • Busch, H
Type
Published Article
Journal
Virchows Archiv. B, Cell pathology including molecular pathology
Publication Date
Jan 01, 1986
Volume
51
Issue
2
Pages
155–160
Identifiers
PMID: 2873678
Source
Medline
License
Unknown

Abstract

Lymphocytes from normal and leukemic patients, and phytohemagglutinin-stimulated lymphocytes were investigated by means of immunofluorescence procedures and a silver reaction for the demonstration of proteins characteristic of nucleolus organizer regions in interphasic cells to provide basic information on the presence of satellite nucleoli in these cells. The results clearly indicated that satellite nucleoli are present in a limited but constant percentage of peripheral lymphocytes. An increased percentage of lymphocytes with satellite nucleoli was found only in leukemic patients and after silver staining. In contrast, a decreased percentage of satellite nucleoli was found 24 h after stimulation with phytohemagglutinin vitro. In leukemic patients, the discrepancy in the percentage of lymphocytes with satellite nucleoli between immunostained and silver-stained preparations may suggest that the silver reaction demonstrates the presence of an additional argyrophilic protein besides proteins B23 and C23, or altered forms of these proteins, which does not react with the specific antibodies.

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