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Studies on Epstein-Barr virus-related antigens. II. Biochemical properties of soluble antigen in Raji Burkitt lymphoma cells.

Authors
  • Matsuo, T
  • Nishi, S
  • Hirai, H
  • Osato, T
Type
Published Article
Journal
International journal of cancer. Journal international du cancer
Publication Date
Mar 15, 1977
Volume
19
Issue
3
Pages
364–370
Identifiers
PMID: 14897
Source
Medline
License
Unknown

Abstract

Biochemical properties of Epstein-Barr virus (EBV)-related soluble antigen in non-producer Raji Burkitt lymphoblastoid cells, assayed by the indirect single radial immunodiffusion (ISRD) test, were investigated. The soluble ISRD antigen retained activity even after exposure to a temperature of 80 degrees C. The antigen was precipitated in 40% saturated ammonium sulfate and ISRD activity was recovered from the precipitate when reconstituted into a solution. Isoelectric focusing and crossed immunoelectrophoretic analyses revealed that the esoelectric point of the ISRD antigen is pH 4.8 with an electrophoretic mobility similar to that of serum alpha-globulin. With Sephadex G-200 or Sepharose 6B gel filtration, ISRD activity was obtained as a single peak which corresponded to the activity absorbing anti-complement immunofluorescence. The molecular weight of the present EBV-related soluble antigen was estimated to be 220,000-2408000 daltons.

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