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Studies on biochemical, oxidative and genotoxicity alterations following vas blockage with reversible inhibition of sperm under guidance and reversal in rats.

Authors
  • Ansari, Abdul S1
  • Hussain, Mubarik2
  • Khan, Sadi Rehan1
  • Badar, Ayesha1
  • Khilwani, Barkha1
  • Lohiya, Nirmal Kumar1
  • 1 Department of Zoology, Centre for Advanced Studies, University of Rajasthan, Sitapura, Jaipur, Rajasthan, India. , (India)
  • 2 Department of Zoology, S. S. Jain Subodh College of Global Excellence, Sitapura, Jaipur, Rajasthan, India. , (India)
Type
Published Article
Journal
Indian journal of pharmacology
Publication Date
Jan 01, 2022
Volume
54
Issue
1
Pages
33–40
Identifiers
DOI: 10.4103/ijp.IJP_557_20
PMID: 35343205
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Vas obstruction with reversible inhibition of sperm under guidance (RISUG) for contraception and its reversal, may cause oxidative stress or inimical effects on male reproductive functions. To evaluate the biochemical and genotoxicity at the level of reactive oxygen species (ROS) following vas occlusion with RISUG and its reversal by Dimethyl sulphoxide (DMSO) and 5% NaHCO3 in Wistar albino rats. Animals were divided into seven groups (n = 10), namely sham-operated control, short-term vas occlusion with RISUG for 90 days, reversal with DMSO and 5% NaHCO3, long-term vas occlusion with RISUG for 360 days, reversal with DMSO and 5% NaHCO3. Biochemical markers in reproductive tissues, hematology, serum biochemistry, serum electrolytes and ROS measuring indicators, e.g., lipid peroxidation, superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase were examined. One-way analysis of variance test was performed for analyses of data obtained in this study using the SPSS 10.0 software (SPSS Inc., Chicago, IL, USA). The tissue and clinical chemistry did not show appreciable alterations in RISUG injected and reversal Groups (II-VII) as compared to sham control. The genotoxicity and various ROS markers fluctuated within control limits following short- and long-term vas occlusion and reversal. The study suggested that the reversal procedures, following RISUG contraception, were not associated with any kind of toxicological manifestations.

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