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Structure of Plant Cell Walls: X. RHAMNOGALACTURONAN I, A STRUCTURALLY COMPLEX PECTIC POLYSACCHARIDE IN THE WALLS OF SUSPENSION-CULTURED SYCAMORE CELLS 1

Authors
  • Michael McNeil
  • Alan G. Darvill
  • Peter Albersheim
Publication Date
Dec 01, 1980
Source
PMC
Keywords
License
Unknown

Abstract

The purification and characterization of a pectic polymer, rhamnogalacturonan I, present in the primary cell walls of dicots is described. Rhamnogalacturonan I accounts for approximately 7% of the mass of the walls isolated from suspension-cultured sycamore cells. As purified, rhamnogalacturonan I has a molecular weight of approximately 200,000 and is composed primarily of l-rhamnosyl, d-galacturonosyl, l-arabinosyl, and d-galactosyl residues. The backbone of rhamnogalacturonan I is thought to be composed predominantly of d-galacturonosyl and l-rhamnosyl residues in a ratio of approximately 2:1. About half of the l-rhamnosyl residues are 2-linked and are glycosidically attached to C4 of a d-galacturonosyl residue. The other half of the l-rhamnosyl residues are 2,4-linked and have a d-galacturonosyl residue glycosidically attached at C2. Sidechains averaging 6 residues in length are attached to C4 of the l-rhamnosyl residues. There are many different sidechains, containing variously linked l-arabinosyl, and/or d-galactosyl residues.

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