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Structure of the human gene for the proliferating cell nuclear antigen.

Authors
  • Travali, S
  • Ku, D H
  • Rizzo, M G
  • Ottavio, L
  • Baserga, R
  • Calabretta, B
Type
Published Article
Journal
The Journal of biological chemistry
Publication Date
May 05, 1989
Volume
264
Issue
13
Pages
7466–7472
Identifiers
PMID: 2565339
Source
Medline
License
Unknown

Abstract

The proliferating cell nuclear antigen (PCNA, cyclin) was originally defined as a nuclear protein whose appearance correlated with the proliferative state of the cell. It is now known to be a co-factor of DNA polymerase delta and to be necessary for DNA synthesis and cell cycle progression. cDNA clones of human PCNA have been isolated and, using one of these cDNA, we have now obtained from a lambda phage library a clone containing the entire human PCNA gene and flanking sequences. The human PCNA gene is a unique copy gene and has 6 exons. It spans, from the cap site to the poly(A) signal 4961 base pairs. We have identified, in the 5'-flanking sequence, a region with promoter activity, a well as other structural elements common to other promoters. An interesting feature of the PCNA gene is the presence of extensive sequence similarities among introns and between introns and exons.

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