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[Structure of DNA complexes with regular polypeptides].

Authors
  • Krylov, A S
  • Gurskiĭ, G V
  • Kondrat'eva, N O
  • Mar'iash, L I
  • Poletaev, A I
Type
Published Article
Journal
Molekuliarnaia biologiia
Publication Date
Jan 01, 1978
Volume
12
Issue
2
Pages
297–307
Identifiers
PMID: 651874
Source
Medline
License
Unknown

Abstract

The conformation of some regular polypeptides: (Lys-Ala)50, (Lys-Ala2)37, (Lys-Ala2)26, (Lys-Ala3)18, (Lys3-Pro)29, (Orn3-Gly)28 was studied by means of CD. The complexes of these polypeptides with DNA were obtained by the methods of jump-dilution of a two-components mixture from 2 M NaCl to 0.05 M NaCl. The extent of DNA covering by the polypeptides was compared using binding isoterms of ethidium on DNA and DNA-polypeptide complex. The length, L, which polypeptides cover on DNA was estimated by means of energy transfer between the dyes absorbed on the complexes. The CD spectra of the complexes revealed a high sensitivity to changes of the environmental conditions. Small variations in the temperature and ionic strength produces marked changes in the CD spectra of the complexes. It was suggested that observed CD changes are due to both the structural relaxation of the complexes and the existence of liquid-crystal domains in solution.

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