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Structure determination of an exopolysaccharide from an alkaliphilic bacterium closely related to Bacillus spp.

Authors
  • Corsaro, M M
  • Grant, W D
  • Grant, S
  • Marciano, C E
  • Parrilli, M
Type
Published Article
Journal
European journal of biochemistry / FEBS
Publication Date
Sep 01, 1999
Volume
264
Issue
2
Pages
554–561
Identifiers
PMID: 10491103
Source
Medline
License
Unknown

Abstract

An exopolysaccharide obtained from an alkaliphilic bacterium closely related to Bacillus spp. was found to contain D-galactopyranuronic acid (GalpA), 2,4-diacetamido-2,4,6-trideoxy-D-glucopyranose (QuipNAc4NAc), 2-acetamido-2-deoxy D-mannopyranuronic acid (ManpNAcA) and one uncommon unit of D-galactopyranuronic acid with the carboxyl group amide-linked to glycine [GalpA(Gly)]. The polysaccharide was studied by one-dimensional and two-dimensional 1H-NMR and 13C-NMR spectroscopy both on native polysaccharide and on monosaccharides and oligosaccharides obtained from methanolysis and from anhydrous HF solvolysis. The following linear structure of the repeating unit was established: -->3)-alpha-D-GalpA(Gly)-(1-->4)-beta-D-ManpNAcA-(1-->4)-alp ha-D-Galp A-(1-->3)-alpha-D-QuipNAc4NAc-(1-->. A preliminary phylogenetic assignment for the bacterium is also reported.

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