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The spliceosome: disorder and dynamics defined

Authors
  • Chen, Weijun
  • Moore, Melissa J1
  • 1 Howard Hughes Medical Institute, Department of Biochemistry and Molecular Pharmacology, RNA and Neuro Therapeutics Institutes, University of Massachusetts Medical School
Type
Published Article
Journal
Current Opinion in Structural Biology
Publisher
Elsevier
Publication Date
Jan 01, 2014
Volume
24
Pages
141–149
Identifiers
DOI: 10.1016/j.sbi.2014.01.009
Source
Elsevier
License
Unknown

Abstract

Among the many macromolecular machines involved in eukaryotic gene expression, the spliceosome remains one of the most challenging for structural biologists. Defining features of this highly complex apparatus are its excessive number of individual parts, many of which have been evolutionarily selected for regions of structural disorder, and the remarkable compositional and conformation dynamics it must undertake to complete each round of splicing. Here we review recent advances in our understanding of spliceosome structural dynamics stemming from bioinformatics, deep sequencing, high throughput methods for determining protein–protein, protein–RNA and RNA–RNA interaction dynamics, single molecule microscopy and more traditional structural analyses. Together, these tools are rapidly changing our structural appreciation of this remarkably dynamic machine.

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