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Structural and expression analyses of two vitellogenin genes in the carp, Cyprinus carpio.

Authors
  • Kang, B J
  • Jung, J-H
  • Lee, J M
  • Lim, S-G
  • Saito, H
  • Kim, M H
  • Kim, Y-J
  • Saigusa, M
  • Han, C-H
Type
Published Article
Journal
Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
Publisher
Elsevier
Publication Date
Dec 01, 2007
Volume
148
Issue
4
Pages
445–453
Identifiers
PMID: 17804271
Source
Medline
License
Unknown

Abstract

We cloned and sequenced two vitellogenin (vg) cDNAs of the carp, Cyprinus carpio, using a cDNA library constructed from estradiol-17 beta (E2)-treated livers. One was a novel, longer 5000 bp-long cDNA termed vg-B2 encoding 1624 amino acids in a single open reading frame. The other was a shorter cDNA (vg-B1), identical to that registered previously as carp vg cDNA in the international nucleotide sequence database. The deduced amino acid sequences of these two molecules were well-aligned with known vertebrate Vgs sharing common characteristics such as N-terminal lipovitellin I (LVI), phosvitin (PV) and C-terminal lipovitellin II (LVII). The novel Vg-B2 bore a highly conserved GL/ICG motif within the LVII region, in contrast to the shorter Vg-B1 that has a truncated C-terminal and lacks the beta-component within the LVII region including the GL/ICG motif. Both vg-B2 and vg-B1 genes were expressed in the livers of females and E2-injected males. Western blot analysis using anti-Vg and anti-vitellin (Vn) antisera demonstrated that both Vg-B2 and Vg-B1 were detected as polypeptides with an estimated molecular mass of 180 kDa and 160 kDa, respectively, in the blood of females and E2-injected males. The results suggest the potential utilization of these genes as sensitive xenoestrogenic markers.

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