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Structural Comparison of Different Galacto-oligosaccharide Mixtures Formed by β-Galactosidases from Lactic Acid Bacteria and Bifidobacteria

Authors
  • Kittibunchakul, Suwapat1, 2
  • van Leeuwen, Sander S.3, 4
  • Dijkhuizen, Lubbert3, 5
  • Haltrich, Dietmar1
  • Nguyen, Thu-Ha1
  • 1 BOKU-University of Natural Resources and Life Sciences, Vienna, Austria , (Austria)
  • 2 Mahidol University, Thailand , (Thailand)
  • 3 University of Groningen, The Netherlands
  • 4 University Medical Center Groningen (UMCG), The Netherlands
  • 5 CarbExplore Research BV, The Netherlands
Type
Published Article
Journal
Journal of Agricultural and Food Chemistry
Publisher
American Chemical Society
Publication Date
Mar 20, 2020
Volume
68
Issue
15
Pages
4437–4446
Identifiers
DOI: 10.1021/acs.jafc.9b08156
PMID: 32196339
PMCID: PMC7168588
Source
PubMed Central
Keywords
License
Unknown

Abstract

The LacLM-type β-galactosidase from Lactobacillus helveticus DSM 20075 expressed in both Escherichia coli ( EcoliBL21Lh β-gal) and Lactobacillus plantarum ( Lp609Lh β-gal) was tested for their potential to form galacto-oligosaccharides (GOS) from lactose. The Lh-GOS mixture formed by β-galactosidase from L. helveticus , together with three GOS mixtures produced using β-galactosidases of both the LacLM and the LacZ type from other lactic acid bacteria, namely, L. reuteri (Lr-GOS), L. bulgaricus (Lb-GOS), and Streptococcus thermophilus (St-GOS), as well as two GOS mixtures (Br-GOS1 and Br-GOS2) produced using β-galactosidases (β-gal I and β-gal II) from Bifidobacterium breve , was analyzed and structurally compared with commercial GOS mixtures analyzed in previous work (Vivinal GOS, GOS I, GOS III, and GOS V) using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), high-performance size-exclusion chromatography with a refractive index (RI) detector (HPSEC-RI), and one-dimensional 1H NMR spectroscopy. β-Galactosidases from lactic acid bacteria and B. breve displayed a preference to form β-(1→6)- and β-(1→3)-linked GOS. The GOS mixtures produced by these enzymes consisted of mainly DP2 and DP3 oligosaccharides, accounting for ∼90% of all GOS components. GOS mixtures obtained with β-galactosidases from lactic acid bacteria and B. breve were quite similar to the commercial GOS III mixture in terms of product spectrum and showed a broader product spectrum than the commercial GOS V mixture. These GOS mixtures also contained a number of GOS components that were absent in the commercial Vivinal GOS (V-GOS).

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