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Structural and Antigenic Characteristics of Streptococcus sobrinus Glucan Binding Proteins

Authors
  • Daniel J. Smith
  • William F. King
  • Christine D. Wu
  • Bella I Shen
  • Martin A. Taubman
Publisher
American Society for Microbiology
Publication Date
Nov 01, 1998
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

Three purified glucan binding proteins (GBP-2, GBP-3, and GBP-5) from Streptococcus sobrinus 6715 were compared structurally by mass spectroscopy of tryptic fragments and antigenically by Western blot analysis with rat antisera to each GBP or to peptides containing putative glucan binding epitopes of mutans streptococcal glucosyltransferases. Structural and antigenic analyses indicated that GBP-3 and GBP-5 are very similar but that both are essentially unrelated to GBP-2. None of these S. sobrinus GBPs appeared to have a strong antigenic relationship with GBPs from Streptococcus mutans. Thus, S. sobrinus GBP-2 and GBP-3 appear to be distinct proteins with potentially different functions. S. sobrinus GBP-5 may be a proteolytic fragment of GBP-3, or, alternatively, the genes coding for these proteins may be closely related.

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