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Strong promoter activity of human and rat islet amyloid polypeptide/amylin gene constructs in mouse beta cells (beta TC 3).

Authors
  • De Wit, L
  • van Mansfeld, A D
  • van Teeffelen, H A
  • Lips, C J
  • Höppener, J W
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Apr 30, 1993
Volume
192
Issue
2
Pages
840–848
Identifiers
PMID: 8484788
Source
Medline
License
Unknown

Abstract

Islet amyloid polypeptide (IAPP)('amylin') is co-produced with insulin in pancreatic beta cells and is the formative polypeptide of pancreatic amyloid in patients with type 2 (non-insulin-dependent) diabetes mellitus. Islet amyloid and type 2 diabetes occur in man, but not in rat. To study transcription regulation of IAPP gene expression in man and rat, luciferase reporter constructs containing different portions of the upstream region of both IAPP genes were expressed in transfected cells. Both the human and the rat IAPP gene constructs revealed higher promoter activity in beta cells (particularly in beta TC3 cells) than in non-beta cells. In both IAPP genes potential transcription elements, with homology to insulin gene transcription elements, were identified. beta TC3 cells provide a good model system in which to study regulation of human and rat IAPP gene expression.

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