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Stress-induced phosphorylation of CLIP-170 by JNK promotes microtubule rescue.

Authors
  • Henrie, Hélène1
  • Bakhos-Douaihy, Dalal1
  • Cantaloube, Isabelle1
  • Pilon, Antoine1, 2
  • Talantikite, Maya1
  • Stoppin-Mellet, Virginie3
  • Baillet, Anita1
  • Poüs, Christian1, 4
  • Benoit, Béatrice1
  • 1 Université Paris-Saclay, Institut National de la Santé et de la Recherche Médicale Unité Mixte de Recherche 1193, Châtenay-Malabry, France. , (France)
  • 2 Département de Biochimie, Hormonologie et Suivi Thérapeutique, Département Médico-Universitaire BioGeM, Assistance Publique - Hôpitaux de Paris Sorbonne Université, Paris, France. , (France)
  • 3 Grenoble Institut des Neurosciences, Institut National de la Santé et de la Recherche Médicale Unité Mixte de Recherche 1216, Université Grenoble Alpes, Grenoble, France. , (France)
  • 4 Biochimie-Hormonologie, Assistance Publique - Hôpitaux de Paris Université Paris-Saclay, Clamart, France. , (France)
Type
Published Article
Journal
The Journal of Cell Biology
Publisher
The Rockefeller University Press
Publication Date
Jul 06, 2020
Volume
219
Issue
7
Identifiers
DOI: 10.1083/jcb.201909093
PMID: 32491151
Source
Medline
Language
English
License
Unknown

Abstract

The stress-induced c-Jun N-terminal kinase (JNK) controls microtubule dynamics by enhancing both microtubule growth and rescues. Here, we show that upon cell stress, JNK directly phosphorylates the microtubule rescue factor CLIP-170 in its microtubule-binding domain to increase its rescue-promoting activity. Phosphomimetic versions of CLIP-170 enhance its ability to promote rescue events in vitro and in cells. Furthermore, while phosphomimetic mutations do not alter CLIP-170's capability to form comets at growing microtubule ends, both phosphomimetic mutations and JNK activation increase the occurrence of CLIP-170 remnants on the microtubule lattice at the rear of comets. As the CLIP-170 remnants, which are potential sites of microtubule rescue, display a shorter lifetime when CLIP-170 is phosphorylated, we propose that instead of acting at the time of rescue occurrence, CLIP-170 would rather contribute in preparing the microtubule lattice for future rescues at these predetermined sites. © 2020 Henrie et al.

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