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Stress response in tardigrades: differential gene expression of molecular chaperones

Authors
  • Reuner, Andy1
  • Hengherr, Steffen1
  • Mali, Brahim2
  • Förster, Frank3
  • Arndt, Detlev4
  • Reinhardt, Richard5
  • Dandekar, Thomas3
  • Frohme, Marcus2
  • Brümmer, Franz1
  • Schill, Ralph O.1
  • 1 Universität Stuttgart, Zoology, Biological Institute, Pfaffenwaldring 57, Stuttgart, 70569, Germany , Stuttgart (Germany)
  • 2 University of Applied Sciences Wildau, Molecular Biology and Functional Genomics, Bahnhofstraße 1, Gebäude 15, Wildau, 15745, Germany , Wildau (Germany)
  • 3 University of Würzburg, Department of Bioinformatics, Biocenter, Am Hubland, Würzburg, 97074, Germany , Würzburg (Germany)
  • 4 European Molecular Biology Laboratory, Developmental Biology Unit, Heidelberg, 69012, Germany , Heidelberg (Germany)
  • 5 MPI for Molecular Genetics, Ihnestrasse 63-73, Berlin-Dahlem, 14195, Germany , Berlin-Dahlem (Germany)
Type
Published Article
Journal
Cell Stress and Chaperones
Publisher
Springer Netherlands
Publication Date
Nov 27, 2009
Volume
15
Issue
4
Pages
423–430
Identifiers
DOI: 10.1007/s12192-009-0158-1
Source
Springer Nature
Keywords
License
Yellow

Abstract

Semi-terrestrial tardigrades exhibit a remarkable tolerance to desiccation by entering a state called anhydrobiosis. In this state, they show a strong resistance against several kinds of physical extremes. Because of the probable importance of stress proteins during the phases of dehydration and rehydration, the relative abundance of transcripts coding for two α-crystallin heat-shock proteins (Mt-sHsp17.2 and Mt-sHsp19.5), as well for the heat-shock proteins Mt-sHsp10, Mt-Hsp60, Mt-Hsp70 and Mt-Hsp90, were analysed in active and anhydrobiotic tardigrades of the species Milnesium tardigradum. They were also analysed in the transitional stage (I) of dehydration, the transitional stage (II) of rehydration and in heat-shocked specimens. A variable pattern of expression was detected, with most candidates being downregulated. Gene transcripts of one Mt-hsp70 isoform in the transitional stage I and Mt-hsp90 in the anhydrobiotic stage were significantly upregulated. A high gene expression (778.6-fold) was found for the small α-crystallin heat-shock protein gene Mt-sHsp17.2 after heat shock. We discuss the limited role of the stress-gene expression in the transitional stages between the active and anhydrobiotic tardigrades and other mechanisms which allow tardigrades to survive desiccation.

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