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StPIP1, a PAMP-induced peptide in potato, elicits plant defenses and is associated with disease symptom severity in a compatible interaction with potato virus Y.

Authors
  • Combest, Max M1, 2
  • Moroz, Natalia3
  • Tanaka, Kiwamu3
  • Rogan, Conner J1
  • Anderson, Jeffrey C1
  • Thura, Lin1, 2
  • Rakotondrafara, Aurélie M4
  • Goyer, Aymeric1, 2
  • 1 Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, USA.
  • 2 Hermiston Agricultural Research and Extension Center, Oregon State University, Hermiston, OR, USA.
  • 3 Department of Plant Pathology, Washington State University, Pullman, WA, USA.
  • 4 Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, USA.
Type
Published Article
Journal
Journal of Experimental Botany
Publisher
Oxford University Press
Publication Date
Mar 03, 2021
Identifiers
DOI: 10.1093/jxb/erab078
PMID: 33681961
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The role of small secreted peptides in plant defense responses to viruses has been seldom investigated. Here, we report a role for potato (Solanum tuberosum) PIP1, a gene predicted to encode a member of the PAMP-induced peptide (PIP) family, in the response of potato to potato virus Y infection (PVY). We show that exogenous application of synthetic StPIP1 to potato leaves and nodes increased the production of reactive oxygen species and the expression of plant defense-related genes, showing that StPIP1 triggers early defense responses. In support of this hypothesis, transgenic potato plants that constitutively overexpress StPIP1 had higher levels of leaf callose deposition, and based on measurements of viral RNA titers, were less susceptible to infection by a compatible PVY strain. Interestingly, systemic infection of StPIP1-overexpressing lines with PVY resulted in clear rugose mosaic symptoms that were absent or very mild in infected non-transgenic plants. A transcriptomics analysis revealed that marker genes associated with both pattern-triggered immunity and effector-triggered immunity were induced in infected StPIP1-overexpressors but not in non-transgenic plants. Together, our results reveal a role for StPIP1 in eliciting plant defense responses and in regulating plant anti-viral immunity. © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: [email protected]

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