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Storage procedures and time influence the detectability of Clostridium difficile toxin A but not toxin B in porcine fecal specimens.

Authors
  • Grześkowiak, Łukasz1
  • Riedmüller, Jonathan1
  • Vahjen, Wilfried1
  • Zentek, Jürgen1
  • 1 Institute of Animal Nutrition, Freie Universität Berlin, Berlin, Germany. , (Germany)
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
Mar 01, 2020
Volume
32
Issue
2
Pages
222–225
Identifiers
DOI: 10.1177/1040638719864383
PMID: 31328697
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Storage procedures are known to affect the detectability of Clostridium difficile toxins in equine and human feces. We assessed the impact of different storage conditions on the detectability of C. difficile toxins in swine feces. Specimens were inoculated with toxins, 112 ng/g of toxin A (TcdA) and 16 ng/g of toxin B (TcdB) and subjected to the following 3 storage treatments: 4°C, -30°C, repetitive freezing at -30°C and thawing. Toxin determination was assessed at 1, 2, 7, 14, and 21 d with ELISA. A decrease in concentrations of TcdA with time was observed for samples stored at 4°C and repetitive freezing-thawing (p ≤0.05). On day 14, storage at 4°C resulted in decreased TcdA concentration as opposed to storage at -30°C and repetitive freezing-thawing (p ≤0.05). On day 21, storage at 4°C resulted in decreased TcdA detectability compared with storage at -30°C (p ≤0.05). The TcdB concentration was unaffected. These results on toxin detectability in swine feces should be carefully considered in in vitro studies on toxigenic C. difficile. Our results also offer valuable information for microbiologists and veterinarians monitoring the presence of virulent C. difficile in pigs.

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