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Stopped-flow--photometric and kinetic--fluorimetric methods for the determination of thyroid hormones in tablets.

Authors
  • Toledano, M
  • Gutiérrez, M C
  • Gómez-Hens, A
  • Pérez-Bendito, D
Type
Published Article
Journal
The Analyst
Publication Date
Feb 01, 1989
Volume
114
Issue
2
Pages
211–215
Identifiers
PMID: 2712319
Source
Medline
License
Unknown

Abstract

The catalytic effect of thyroxine and triiodothyronine on the reaction between cerium(IV) and arsenic(III) was studied by using photometric and fluorimetric measurements. The kinetic - photometric studies were based on measurement of the decrease in absorbance of cerium(IV), whereas the rate of formation of fluorescent cerium(III) was measured in the kinetic - fluorimetric studies. A modular stopped-flow system was coupled to a spectrophotometer in order to develop stopped-flow methods for the determination of thyroxine and triiodothyronine. The proposed methods were compared and applied to the determination of these hormones in pharmaceutical preparations.

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