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Stimulus-Responsive Regulation of Enzyme Activity for One-Step and Multi-Step Syntheses.

Authors
  • Claaßen, Christiane1
  • Gerlach, Tim1, 2
  • Rother, Dörte1, 2
  • 1 Institute of Bio- and Geosciences - Biotechnology (IBG-1) Forschungszentrum Jülich GmbH 52425 Jülich Germany. , (Germany)
  • 2 Aachen Biology and Biotechnology (ABBt) RWTH Aachen University 52074 Aachen Germany. , (Germany)
Type
Published Article
Journal
Advanced Synthesis & Catalysis
Publisher
Wiley
Publication Date
Jun 06, 2019
Volume
361
Issue
11
Pages
2387–2401
Identifiers
DOI: 10.1002/adsc.201900169
PMID: 31244574
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Multi-step biocatalytic reactions have gained increasing importance in recent years because the combination of different enzymes enables the synthesis of a broad variety of industrially relevant products. However, the more enzymes combined, the more crucial it is to avoid cross-reactivity in these cascade reactions and thus achieve high product yields and high purities. The selective control of enzyme activity, i.e., remote on-/off-switching of enzymes, might be a suitable tool to avoid the formation of unwanted by-products in multi-enzyme reactions. This review compiles a range of methods that are known to modulate enzyme activity in a stimulus-responsive manner. It focuses predominantly on in vitro systems and is subdivided into reversible and irreversible enzyme activity control. Furthermore, a discussion section provides indications as to which factors should be considered when designing and choosing activity control systems for biocatalysis. Finally, an outlook is given regarding the future prospects of the field.

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