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Stimulation of human nitric oxide synthase by tetrahydrobiopterin and selective binding of the cofactor.

Authors
  • Klatt, P
  • Heinzel, B
  • Mayer, B
  • Ambach, E
  • Werner-Felmayer, G
  • Wachter, H
  • Werner, E R
Type
Published Article
Journal
FEBS Letters
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jun 29, 1992
Volume
305
Issue
2
Pages
160–162
Identifiers
PMID: 1377649
Source
Medline
License
Unknown

Abstract

To check the stimulatory potency of the tetrahydro forms of the two major pteridines occurring in human tissues, neopterin and biopterin, NO synthase was purified 6000-fold from human cerebellum. Tetrahydrobiopterin stimulated the activity up to 4.5-fold in a concentration dependent manner with a maximum above 1 microM, whereas tetrahydroneopterin was completely inactive in concentrations up to 100 microM. Tetrahydrobiopterin, but not neopterin derivatives, were copurified with the NO synthase activity. Our results demonstrate that human cerebellum contains a tetrahydrobiopterin dependent NO synthase activity.

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