Affordable Access

Sth132I, a novel class-IIS restriction endonuclease of Streptococcus thermophilus ST132.

Authors
  • Poch, M T
  • Somkuti, G A
  • Solaiman, D K
Type
Published Article
Journal
Gene
Publisher
Elsevier
Publication Date
Aug 22, 1997
Volume
195
Issue
2
Pages
201–206
Identifiers
PMID: 9305765
Source
Medline
License
Unknown

Abstract

The Sth132I restriction endonuclease (R.Sth132I) was detected in Streptococcus thermophilus ST132 and purified to near homogeneity by heparin Sepharose CL-6B affinity chromatography. Fragments from Sth132I digestion of plasmid DNA were subcloned into pUC19 in Escherichia coli DH5alpha and sequenced. Sequence analysis of inserts and their ligation junction sites revealed that Sth132I is a novel class-IIS restriction endonuclease, which recognizes the non-palindromic sequence 5'-CCCG(N)4-3', 3'-GGGC(N) 8-5'.

Report this publication

Statistics

Seen <100 times