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Starch gel electrophoresis of lactate dehydrogenase and glucose phosphate isomerase of poultry coccidia using the LKB multiphor.

Authors
  • Kucera, J
Type
Published Article
Journal
Folia parasitologica
Publication Date
Jan 01, 1989
Volume
36
Issue
4
Pages
295–299
Identifiers
PMID: 2488045
Source
Medline
License
Unknown

Abstract

A modification of thin-layer starch gel horizontal electrophoresis is described. The original method of Wraxall and Culliford (1986) is improved so that the preparation of starch gel is as simple as preparing the agarose gel. Thus the commercially supplied kits and instruments for the agarose gel electrophoresis can be also used for the starch gel electrophoresis. Furthermore, a method of preparing the permanent dry enzymograms from the starch gels with visualized enzymes is presented. The described procedure was used in the LDH and GPI analyses of poultry coccidia.

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