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Standardized Xylocarpus moluccensis fruit fraction mitigates collagen-induced arthritis in mice by regulating immune response.

Authors
  • Gupta, Priya1
  • Kumar, Amit1
  • Pal, Subhashis2
  • Kumar, Sachin1
  • Lahiri, Amit1
  • Kumaravelu, Jagavelu1
  • Chattopadhyay, Naibedya2
  • Dikshit, Madhu1
  • Barthwal, Manoj Kumar1
  • 1 Division of Pharmacology, Council of Scientific and Industrial Research-Central Drug Research Institute (CSIR-CDRI), Lucknow, India. , (India)
  • 2 Division of Endocrinology, Council of Scientific and Industrial Research-Central Drug Research Institute (CSIR-CDRI), Lucknow, India. , (India)
Type
Published Article
Journal
The Journal of pharmacy and pharmacology
Publication Date
Apr 01, 2020
Volume
72
Issue
4
Pages
619–632
Identifiers
DOI: 10.1111/jphp.13231
PMID: 32037560
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This study was undertaken to evaluate the effect of Xylocarpus moluccensis fruit fraction (F018) on the pathogenesis of collagen-induced arthritis in mice. Arthritis was induced by intradermal injection of collagen (2 mg/ml) with complete Freund's adjuvant in DBA/1J mice. F018 was administered orally at 1, 3 and 10 mg/kg for 20 days. Disease progression and mechanism were assessed by micro-CT analysis, RT-PCR, flow cytometry assay, myeloperoxidase (MPO) and MTT assay. F018 at 3 and 10 mg/kg significantly reduced paw thickness, clinical score, mononuclear cell infiltration and collagen layer depletion in the knee section of collagen-induced arthritis (CIA) mice when compared with collagen-induced arthritis mice alone. Furthermore, F018 treatment in collagen-induced arthritis mice significantly recovered bone volume and trabecular number and decreased the trabecular space by modulating RANKL and OPG mRNA expression in the synovial tissue. F018 treatment in collagen-induced arthritis mice significantly attenuated spleen index, lymphocyte proliferation and paw myeloperoxidase (MPO) activity, pro-inflammatory cytokine TNFα, IL1β, and IL6 mRNA expression and enhanced IL10 mRNA expression in paw tissue. Furthermore, F018 treatment in collagen-induced arthritis mice significantly reduced splenic dendritic cell maturation and Th17 cells. In culture, F018 significantly decreased collagen-induced arthritis-FLS proliferation and promoted apoptosis. F018 may serve as a potential curative agent for arthritis. © 2020 Royal Pharmaceutical Society.

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